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Polysaccharide-based silver nanoparticles synthesized by Klebsiella oxytoca DSM 29614 cause DNA fragmentation in E. coli cells

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Abstract

Silver nanoparticles (AgNPs), embedded into a specific exopolysaccharide (EPS), were produced by Klebsiella oxytoca DSM 29614 by adding AgNO3 to the cultures during exponential growth phase. In particular, under aerobic or anaerobic conditions, two types of silver nanoparticles, named AgNPs-EPSaer and the AgNPs-EPSanaer, were produced respectively. The effects on bacterial cells was demonstrated by using Escherichia coli K12 and Kocuria rhizophila ATCC 9341 (ex Micrococcus luteus) as Gram-negative and Gram-positive tester strains, respectively. The best antimicrobial activity was observed for AgNPs-EPSaer, in terms of minimum inhibitory concentrations and minimum bactericidal concentrations. Observations by transmission electron microscopy showed that the cell morphology of both tester strains changed during the exposition to AgNPs-EPSaer. In particular, an electron-dense wrapped filament was observed in E. coli cytoplasm after 3 h of AgNPs-EPSaer exposition, apparently due to silver accumulation in DNA, and both E. coli and K. rhizophila cells were lysed after 18 h of exposure to AgNPs-EPSaer. The DNA breakage in E. coli cells was confirmed by the comparison of 3-D fluorescence spectra fingerprints of DNA. Finally the accumulation of silver on DNA of E. coli was confirmed directly by a significant Ag+ release from DNA, using the scanning electrochemical microscopy and the voltammetric determinations.

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Acknowledgments

Financial support of the Ministry of University and Scientific Research (MIUR) (PRIN-2010AXENJ8) and of Cà Foscari University (ADIR) are gratefully acknowledged.

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Authors and Affiliations

  1. Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari of Venice, Via Torino 155, 30172, Venice, Italy

    Franco Baldi, Salvatore Daniele, Michele Gallo & Stefano Paganelli

  2. Dipartimento di Scienze Ambientali, Infomatica e Statistica, University Cà Foscari of Venice, Via Torino 155, 30172, Venice, Italy

    Dario Battistel

  3. SCSOP, Via Bornò 5, 23896, Sirtori, LC, Italy

    Oreste Piccolo

  4. Dipartimento Scienze della Vita, University of Siena, Via Mattioli 4, 53100, Siena, Italy

    Claudia Faleri

  5. Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Viale delle Scienze - ed. 16, 90128, Palermo, Italy

    Anna Maria Puglia & Giuseppe Gallo

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  1. Franco Baldi
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  2. Salvatore Daniele
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Correspondence to Franco Baldi.

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Baldi, F., Daniele, S., Gallo, M. et al. Polysaccharide-based silver nanoparticles synthesized by Klebsiella oxytoca DSM 29614 cause DNA fragmentation in E. coli cells. Biometals 29, 321–331 (2016). https://doi.org/10.1007/s10534-016-9918-4

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