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Trichoderma harzianum strain T-22 induces changes in phytohormone levels in cherry rootstocks (Prunus cerasus × P. canescens)

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Abstract

The aim of this research was to explain the direct plant growth-promoting activity of Trichoderma harzianum strain T-22 (T22), hypothesizing the involvement of different classes of plant growth regulators. Seven days after the transfer to root-inducing medium, in vitro-cultured shoots of GiSeLa6® (Prunus cerasus  × P. canescens) were inoculated with T22. Root and shoot growth were significantly affected by T22 (+76 and +61%, respectively). Ten days after inoculation, the levels of indole-3-acetic acid (IAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromatography coupled with mass spectrometry. The results showed that after T22-inoculation, IAA and GA3 significantly increased in both leaves (+49 and +71%, respectively) and roots (+40 and +143%, respectively) whereas t-ZR decreased (−51% in leaves and −37% in roots). Changes in DHZR were observed in T22-inoculated roots (−32%) but not in leaves, whereas the levels of ABA did not differ between the two treatments. The extraction method allowed the simultaneous extraction of phytohormones. There is evidence that the change in phytohormone levels is one of the direct mechanism by which T22 promotes rooting and shoot growth, with notable advantages for rootstock production during nursery processes.

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Fig. 1
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Abbreviations

DHZR:

Dihydrozeatin riboside

GA3:

Gibberellic acid

IAA:

Indole-3-acetic acid

t-ZR:

trans-zeatin riboside

T22:

Trichoderma harzianum strain T-22

References

  • Blake PS, Browning G, Benjamin LJ, Mander LN (2000) Gibberellins in seedlings and flowering trees of Prunus avium L. Phytochemistry 53:519–528

    Article  CAS  PubMed  Google Scholar 

  • Contreras-Cornejo HA, Macías-Rodríguez L, Cortés-Penagos C, López-Bucio J (2009) Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in arabidopsis. Plant Physiol 149:1579–1592

    Article  CAS  PubMed  Google Scholar 

  • Harman GE, Lorito M, Lynch JM (2004) Uses of Trichoderma spp. to alleviate or remediate soil and water pollution. In: Laskin AI, Bennett JW, Gadd GM (eds) Advances in applied microbiology. vol 56. Elsevier, San Diego, pp 313–330

    Chapter  Google Scholar 

  • Hedden P, Thomas SG (2006) Plant hormone signaling. Blackwell, Oxford, pp 37–66

    Book  Google Scholar 

  • Kleifeld O, Chet I (1992) Trichoderma harzianum -interaction with plants and effect on growth response. Plant Soil 144:267–272

    Article  Google Scholar 

  • Kobashi K, Sugaya S, Gemma H, Iwahori S (2001) Effect of abscisic acid (ABA) on sugar accumulation in the flesh tissue of peach fruit at the start of the maturation stage. Plant Growth Regul 35:215–223

    Article  CAS  Google Scholar 

  • Miyadera H, Shiomi K, Ui H, Yamaguchi Y, Masuma R, Tomoda H, Miyoshi H, Osanai A, Kita K, Ōmura S (2003) Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase). Proc Natl Acad Sci USA 100:473–477

    Article  CAS  PubMed  Google Scholar 

  • Moncaleán P, Rodríguez A, Fernández B (2001) Plant growth regulators as putative physiological markers of developmental stage in Prunus persica. Plant Growth Regul 36:27–29

    Article  Google Scholar 

  • Singh V, Singh PN, Yadav RL, Awasthi SK, Joshi BB, Singh RK, Lal RJ, Duttamajumder SK (2010) Increasing the efficacy of Trichoderma harzianum for nutrient uptake and control of red rot in sugarcane. J Hortic For 2:66–71

    Google Scholar 

  • Sofo A, Milella L, Tataranni G (2010) Effects of Trichoderma harzianum strain T-22 on the growth of two Prunus rootstocks during the rooting phase. J Hortic Sci Biotechnol 85:497–502

    Google Scholar 

  • Sorce C, Mariotti L, Lorenzi R, Massai R (2007) Hormonal factors involved in the control of vigour of grafted peach [Prunus persica (L.) Batsch] trees and hybrid rootstocks. Adv Hortic Sci 21:68–74

    Google Scholar 

  • Srivastava LM (2002) Plant, growth and development-hormones and environment. Elsevier, San Diego, pp 307–314

    Google Scholar 

  • Tworkoski T, Miller S, Scorza R (2006) Relationship of pruning and growth morphology with hormone ratios in shoots of pillar and standard peach trees. J Plant Growth Regul 25:145–155

    Article  CAS  Google Scholar 

  • Windham MT, Elad Y, Baker R (1986) A mechanism for increased plant growth induced by Trichoderma spp. Phytopathology 76:518–521

    Article  Google Scholar 

  • Yang H–H, Yang SL, Peng K-C, Lo CT, Liu S-Y (2009) Induced proteome of Trichoderma harzianum by Botrytis cinerea. Mycol Res 113:924–932

    Article  CAS  PubMed  Google Scholar 

  • Yedidia I, Srivastva AK, Kapulnik Y, Chet I (2001) Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil 235:235–242

    Article  CAS  Google Scholar 

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Correspondence to Adriano Sofo.

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Sofo, A., Scopa, A., Manfra, M. et al. Trichoderma harzianum strain T-22 induces changes in phytohormone levels in cherry rootstocks (Prunus cerasus × P. canescens). Plant Growth Regul 65, 421–425 (2011). https://doi.org/10.1007/s10725-011-9610-1

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  • DOI: https://doi.org/10.1007/s10725-011-9610-1

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