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Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response

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Abstract

It is well known that human keratinocytes produce the anti-microbial peptide β-defensin 2. Its production is enhanced by pathogenic microorganisms or other environmental stressors. In this study, we evaluated the effect of resveratrol, a polyphenol found in several dietary source as grape seed, and its natural precursor, polydatin on heat-stressed human keratinocytes. By reverse transcription-polymerase chain reaction and enzyme-linked immunoadsorbent assay, we demonstrated that resveratrol used in combination with polydatin was able to modulate interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha gene expression. In addition, our data show that resveratrol and polydatin increased the heat shock protein (Hsp)70B′ gene expression, a Hsp that plays an important role in the cytoprotection and repair of cells and tissues. Worthy of note, polydatin used alone or in combination with resveratrol, increased the release of human β-defensin 2. These results highlighted the ability of polydatin and resveratrol to reinforce cytoprotective response in stress conditions and suggest their use in cosmetic or pharmaceutical preparations.

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ACKNOWLEDGMENTS

This work was supported by grants from GLURES, s.r.l. Academic SPIN-OFF Ca’ Foscari University of Venice, Italy.

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Authors and Affiliations

  1. GLURES, Academic SPIN-OFF, Ca’ Foscari University of Venice, Venice, Italy

    Giampietro Ravagnan & Salvatore De Maria

  2. Section of Microbiology and Clinical Microbiology, Department of Experimental Medicine, Second University of Naples (SUN), via Luigi de Crecchio n° 7, 80138, Naples, Italy

    Anna De Filippis, Marcella Petrazzuolo, Maria Antonietta Tufano & Giovanna Donnarumma

  3. Section of Biotechnology, Department of Experimental Medicine, Second University of Naples, Naples, Italy

    Maria Cartenì

  4. Department of Mathematics and Statistics, University Study of Naples “Federico II”, Naples, Italy

    Valentina Cozza

Authors
  1. Giampietro Ravagnan
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  2. Anna De Filippis
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  3. Maria Cartenì
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  4. Salvatore De Maria
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  5. Valentina Cozza
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  6. Marcella Petrazzuolo
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  7. Maria Antonietta Tufano
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  8. Giovanna Donnarumma
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Corresponding author

Correspondence to Maria Antonietta Tufano.

Additional information

Ravagnan Giampietro and De Filippis Anna contributed equally to this work.

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Ravagnan, G., De Filippis, A., Cartenì, M. et al. Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response. Inflammation 36, 26–34 (2013). https://doi.org/10.1007/s10753-012-9516-8

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  • DOI: https://doi.org/10.1007/s10753-012-9516-8

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