Improved enzymatic hydrolysis of hair
References (4)
- et al.
Drugs in hair: a new extraction procedure
Forensic Sci. Int.
(1989) - et al.
Radioimmunoassay of hair for determining opiate-abuse histories
J. Nucl. Med.
(1979)
Cited by (25)
Matrix solid phase dispersion assisted enzymatic hydrolysis as a novel approach for cocaine and opiates isolation from human hair
2013, Journal of Chromatography ACitation Excerpt :A second set of experiments were conducted to observe the effect of DTT during the enzymatic hydrolysis of hair. DTT is commonly used to enhance the enzymatic activity of Pronase E. Its action consists in breaking down certain disulphide bonds of the keratin matrix, allowing a faster action of the enzyme [50]. Fig. 1(b) shows results in triplicate obtained for MSPD-EH experiments and elution with 5 mL of a solution containing 2.0 mg mL−1 Pronase E and 12.5 mg mL−1 DTT in 1.4 M/1.4 M Tris/HCl, pH 7.3 (MSPD-EH with DTT), or with 5 mL of 2.0 mg mL−1 Pronase E in 1.4 M/1.4 M Tris/HCl, pH 7.3 (MSPD-EH without DTT).
Matrix solid-phase dispersion on column clean-up/pre-concentration as a novel approach for fast isolation of abuse drugs from human hair
2010, Journal of Chromatography ACitation Excerpt :Reviews on this topic show numerous extraction treatments mainly based on methanol, aqueous acids or buffer solutions as extracting solvents, alkaline digestions with aqueous sodium hydroxide, or enzymatic digestions (hydrolysis) [1,11,12]. Methods based on acidic hydrolysis have been reported to offer high yields for cocaine, opiates and their metabolites [13], although enzymatic hydrolysis are also recommended, mainly because the moderate pH and temperature conditions inherent to these procedures [14–19]. In addition, the possibility of speeding up the enzymatic hydrolysis procedures by using ultrasound irradiation has offered important practical advantages for shortening the whole analytical procedure [20].
State of the art in hair analysis for detection of drug and alcohol abuse
2006, Clinica Chimica ActaHair analysis for veterinary drug monitoring in livestock production
2006, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesSolid-phase microextraction for the determination of cocaine and cocaethylene in human hair by gas chromatography-mass spectrometry
2006, Forensic Science InternationalScreening for drugs of abuse in hair with ion spray LC-MS-MS
2004, Forensic Science International