Elsevier

Results in Optics

Volume 5, December 2021, 100123
Results in Optics

Invited Article
(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance

https://doi.org/10.1016/j.rio.2021.100123Get rights and content
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open access

Highlights

  • Small target analyte detection with nano-film coated D-shaped fiber.

  • Perfluorooctanoic acid detection via lossy mode resonance phenomenon.

  • Label-free optical sensing strategies based-on delipidated human serum albumin.

Abstract

The determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among them, lossy mode resonance (LMR) fiber optic biosensors are showing remarkable performance in terms of detection limit, selectivity, and reproducibility. The detection of small molecules, such as perfluorooctanoic acid (PFOA), can be achieved with the help of well-designed biological recognition layers. In this study, the biosensing potentialities of a label-free LMR-assisted optical platform based on nanocoated fibers are investigated. Delipidated human serum albumin (hSA) was used as biological recognition layer for PFOA in aqueous solution. Different fiber functionalization protocols based on the covalent immobilization of hSA were tested. The conformational changes related to the formation of hSA/PFOA complex were followed via optical monitoring of LMR spectral shift, showing a trend that can be modeled with Langmuir adsorption isotherm. These results confirmed the potentiality of LMR-based fiber biosensors for the detection of small molecules, such as PFOA, in synthetic samples.

Keywords

Nanocoated fiber sensor
Lossy mode resonance
Small target detection
Delipidated human serum albumin
Perfluorooctanoic acid
Label-free biosensing

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