Issue 4, 2011
From the journal:

Analytical Methods

SERSlabels for quantitative assays: application to the quantification of gold nanoparticles uptaken by macrophage cells

Vincenzo Amendola,*a   Moreno Meneghetti,a   Stefania Fiameni,b   Stefano Polizzi,c   Giulio Fracasso,d   Anita Boscainid  and  Marco Colombattid  

* Corresponding authors

a Department of Chemical Sciences, University of Padova, via Marzolo 1, Padova, Italy
E-mail: vincenzo.amendola@unipd.it

b CNR—IENI, Corso Stati Uniti 4, Padova, Italy

c Department of Molecular Sciences and Nanosystems, Università Ca' Foscari Venezia and INSTM UdR Venezia, via Torino 155/b, Venezia-Mestre, Italy

d Department of Pathology and Diagnostic, Section of Immunology, University of Verona, P.le LA Scuro 10, Verona, Italy

Abstract

Labels based on noble metal nanoparticles and surface enhanced Raman scattering (SERS) opened new opportunities for the ultrasensitive detection of analytes. To date, however, SERS labels were mostly used for qualitative analysis, while leaving largely unexploited their potential for ultrasensitive quantitative assays. Here we synthesized SERS labels based on gold nanoparticles (AuNPs) obtained by laser ablation synthesis in solution and we developed a general method for the correlation of the SERS label concentration with the intensity of the Raman signal. We successfully used this method for the quantification of the number of AuNPs uptaken by PMA differentiated U937 macrophage cells. Our work shows that quantitative ultrasensitive assays by SERS labels are possible and points out some issues that must be considered when performing this type of analysis.

Graphical abstract: SERS labels for quantitative assays: application to the quantification of gold nanoparticles uptaken by macrophage cells

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C0AY00660B
Article type
Paper
Submitted
01 Nov 2010
Accepted
28 Jan 2011
First published
24 Feb 2011

Anal. Methods, 2011,3, 849-856

Permissions

Request permissions

SERS labels for quantitative assays: application to the quantification of gold nanoparticles uptaken by macrophage cells

V. Amendola, M. Meneghetti, S. Fiameni, S. Polizzi, G. Fracasso, A. Boscaini and M. Colombatti, Anal. Methods, 2011, 3, 849 DOI: 10.1039/C0AY00660B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements